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When using IEM, i5 sequences needs to be entered into these program from the forward orientation in all circumstances and for all instrument styles.
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Make reference to Index two (i5) Orientation For more info on how to enter i5 bases to the sample sheet in ahead or reverse complement orientation.
When demultiplexing, the ultimate sample sheet furnished to your FASTQ technology software program really should provide the i5 index in the correct orientation for that platform. However, most Illumina sample sheet generation application handles this immediately.
Create full-genome sequencing libraries and successfully interrogate samples with restricted out there DNA.
Set C Index Adapters Seek advice from Index 2 (i5) Orientation To find out more regarding how to enter i5 bases on the sample sheet in ahead or reverse enhance orientation.
Established D Index Adapters Make reference to Index two (i5) Orientation To learn more on how to enter i5 bases to the sample sheet in forward or reverse complement orientation.
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Library prep, enrichment, and probe panel reagents have to be requested independently. Compatible library prep kits: